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How should suspension cells be passaged?
Publication date：2026-10-29
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Typically, you simply need to continuously add fresh culture medium to the original flask to dilute the cell concentration. If the volume of culture medium becomes excessive, gently elevate the opening of the flask until it can no longer accommodate the liquid. When passaging, transfer a portion of the cell-containing medium into a new flask, add fresh medium to bring the concentration to an appropriate level, and then repeat the preceding steps.
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