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Original article link: HeYuan Bio – Autophagy
Autophagy is a cellular process in which cells degrade their own components via the lysosomal degradation pathway, serving as a major metabolic pathway that enables cell survival under stress conditions such as nutrient deprivation. Autophagy is broadly classified into three main types: macroautophagy, microautophagy, and chaperone-mediated autophagy. It plays a critical role in a variety of diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
The process of autophagy proceeds as follows:
Figure 1: The process of autophagy
Autophagy Product Introduction:
1. Autophagy screening system: GFP-LC3 WT/MT, GFP-LC3-RFP-LC3 MT, Luciferase LC3 WT/Luciferase LC3 MT
Figure 2: Autophagy screening system
2. Autophagy detection system:
Single-fluorescent LC3 virus: pLenti-CMV-EGFP-LC3-PGK-Puro, pLenti-Ubc-EGFP-3FLAG-LC3, pLenti-EF1a-GFP-LC3-PGK-Puro
Dual-fluorescent LC3 virus: AdV-mCherry-GFP-LC3 (high-efficiency infection), LV-mCherry-GFP-LC3 (stable expression), AAV-mCherry-GFP-LC3 (in vivo detection); Western blot analysis of LC3 I/II and p62; LC3 and p62 provided as antibodies for detection.
Figure 3 Autophagy detection system
3. Lentiviral vectors for autophagy gene overexpression and knockdown:
Lentiviral interference, 3 targets/genes, with an interference efficiency of over 70%
Lentiviral overexpression, eukaryotic expression vector ORF cloning, and corresponding detection antibodies
Figure 4: Autophagy Gene Manipulation
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