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How should suspension cells be passaged?
Publication date：2026-10-29
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Typically, you simply need to continuously add fresh culture medium to the original culture flask and dilute the cell concentration. If the volume of culture medium becomes excessive, slightly elevate the opening of the flask until it can no longer accommodate the liquid. When subculturing, transfer a portion of the cell-containing medium into a new culture flask, add fresh medium to bring the cell density to an appropriate level, and then repeat the preceding steps.
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