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How should cell debris generated during culture be handled?
Publication date:2026-10-29
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After removing the original culture medium, adhere‑dependent cells should be rinsed 2–3 times with PBS; for suspension‑dependent cells, centrifuge to remove the original medium, then resuspend in PBS and centrifuge again to remove the PBS. It is generally recommended to repeat these steps 2–3 times, followed by centrifugation at 800–1000 rpm for 3–5 minutes.
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