“He Yuan Li Ji” was established as a joint venture between He Yuan Bio (stock code: 688238) and Li Ji Bio, with its core business centered on the “Li Ji Bio” and “Life-ilab” reagent brands.

Select language:

中文

English

What is the formula for calculating cell viability in CCK‑8 assays?

Publication date:2026-10-29
Page views:

Cell viability assay: Cell viability (%) = [A (with drug) – A (blank)] / [A (0 drug) – A (blank)] × 100.

[Note 】 A (Drug Addition): The absorbance of the well containing cells, CCK‑8 solution, and drug solution.

A (blank): The absorbance of a well containing culture medium and CCK-8 solution but no cells.

A (0 drug addition): The absorbance of wells containing cells and CCK‑8 solution but no drug solution.