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What is the transfection efficiency of single-plasmid versus co-transfection of multiple plasmids?
Publication date：2026-10-29
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The transfection efficiency of a single plasmid is generally excellent for validated cell lines; you can refer to these established cell lines. For co-transfection, however, since it involves both the mixing ratio of plasmids and the ratio of plasmid to transfection reagent, the actual efficiency must be determined through appropriate validation.
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