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Before the experiment, should we first test whether the culture medium and CCK‑8 reagent react with each other?
Publication date：2026-10-29
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It is necessary to perform a preliminary test using a single well, as the culture medium may contain substances that participate in redox reactions. Before conducting the formal experiment, it is essential to verify whether the medium reacts with CCK‑8. Typically, the OD value under normal conditions should be below 0.4.
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