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Why is it necessary to collect the cell supernatant in the Annexin V‑PI assay?
Publication date:2026-10-29
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Because apoptotic cells may detach from the culture dish and remain suspended in the culture medium, it is necessary to collect the supernatant, centrifuge it to pellet the cells, and combine these with the cells released by trypsinization; otherwise, the measured proportion of apoptosis may be underestimated.
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