“He Yuan Li Ji” was established as a joint venture between He Yuan Bio (stock code: 688238) and Li Ji Bio, with its core business centered on the “Li Ji Bio” and “Life-ilab” reagent brands.

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Half-Price New Arrival | Limited-Time Offer on Column-Based Protein Extraction Kits, Plus a Big Sale on the WB Series

Publication date:2024-04-10
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Li Ji Bio’s new, highly effective product has been added—yet again! This time, we’re launching… AP01L204 – Column-Based Total Protein Extraction Kit for Animal Tissues and Cells The extraction process takes only 2 minutes, making it an outstanding tool for total protein isolation! (New product—half price—see details below.)

Buy WB series products now and enjoy Limited-time buy-one-get-one-free offer, plus a stylish coffee mug and Huawei Bluetooth earphones. Enjoy exclusive gifts! (The threshold is extremely low—see the end of the article for details.)

  • Part 1. Product Overview

Column-Based Animal Tissue/Cell Total Protein Extraction Kit

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This kit innovatively employs a column‑based purification method, enabling rapid, gentle, and efficient lysis of animal tissues or cells to effectively extract total protein. It includes both a spin column and an optimized cell lysis buffer. Compared with conventional RIPA lysis buffers, it yields higher protein recovery and minimizes the loss of certain proteins caused by DNA precipitation and insoluble cellular debris during cell lysis. Utilizing column‑based purification, the system can process sample–lysis buffer mixtures ranging from as little as 20 μL to as much as 500 μL. Two types of cell lysis buffers are provided—denaturing and non‑denaturing—allowing users to select the appropriate buffer based on their downstream experimental requirements.

● ●   Product Advantages

Quick: The extraction process takes only 2 minutes and requires no ice‑based handling.

Convenience: Simply centrifuge; no sonication required.

General: Suitable for all types of animal cells and tissues.

Efficient: No protein loss, making it particularly suitable for the extraction of small-molecule proteins, membrane proteins, nucleic acid‑binding proteins, and phosphorylated proteins.

● ●   Experimental Procedure

● ● Experimental data

Figure 1. During extraction, RIPA lysis buffer results in protein loss, with substantial protein remaining in the insoluble pellet—particularly for low‑molecular‑weight proteins—whereas the total protein extraction kit yields no detectable protein loss.


Figure 2. The column-based whole-protein extraction kit demonstrates significantly superior performance in isolating membrane proteins, phosphorylated proteins, and transcription factors compared to RIPA lysis buffer.

  • Part 2. Limited-Time Event

① New arrivals—half price with passion!

AP01L204 – Column-Based Total Protein Extraction Kit for Animal Tissues and Cells, Original price: 898 yuan , Limited-time half-price: 449 yuan !

② Big sale on WB series products

Buy now and enjoy a buy-one-get-one-free offer on the WB series, plus exclusive gifts like a stylish coffee mug and Huawei Bluetooth earphones!

This event ends on May 19—don’t miss out! See the list of participating products below:

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First look at the free gift with purchase ↓↓

✔ Stylish coffee mug ✔ “Every bet wins” tote strap

✔ Huawei “Far Ahead” ✔ Premium Sound Quality

③ Rules for this event

Event Time: Effective immediately–May 19, 2024

Scope of Activities: National bio-related end customers

Purchase method: Please contact the regional distributors and sales representatives.

Precautions:

1. Follow the WeChat official account to participate in the event;

2. All gifts are shipped in random colors from the warehouse.

3. The final interpretation of this event rests with Heyuan Liji.

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