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Introduction to Tissue Dissociation Solutions and Instructions for Use
Tissue dissociation solution is a mixture of various biological enzymes that gently, rapidly, and efficiently enzymatically digest the extracellular matrix of tissues, releasing cells to form a cell suspension. It is suitable for isolating cells from a wide range of tissues, including the spleen, liver, kidneys, pancreas, gastric mucosa, gallbladder, and tumor tissues.
[Product] Tissue dissociation solution
[Item Number] AC05L322
[Specification] 10 mL
Product Advantages
• Suitable for Human-derived and animal-derived Various organizations;
• Short turnaround time; no specialized equipment required. ；
• Obtained High cell viability , can be retained Cell surface antigen ；
• Cells are used for Cell culture, cell sorting, drug screening, single-cell sequencing Wait;
• Store at -20℃; shelf life: 6 months.
Experimental parameters
Experimental Procedure
Reagents: Cell culture medium, red blood cell lysis buffer (Catalog No.: AP01L106) (optional)
Consumables: Centrifuge tube, disposable sterile filter (70 µm)
Equipment: Ophthalmic scissors, ophthalmic forceps
Instrument: Constant-temperature shaker, centrifuge
Instructions for Use
1. Weigh approximately 100 mg of fresh tissue and transfer it to a sterile centrifuge tube. Using ophthalmic scissors, mince the tissue thoroughly into pieces measuring 1–2 mm³, then add 1 mL of tissue digestion solution.
2. Place the centrifuge tube on a constant-temperature shaker and incubate at 37°C with shaking at 180 rpm for 10–30 minutes to digest the tissue; the digestion time may be shortened or extended as appropriate depending on the type of tissue.
3. After incubation, filter the tissue homogenate in the centrifuge tube through a 70 µm sterile filter, then add cell culture medium to rinse the filter and collect the filtrate in the centrifuge tube.
4. Centrifuge the cell suspension at 1200 rpm for 5 minutes, and discard the supernatant.
5. Add an appropriate volume of culture medium, centrifuge at 1200 rpm for 5 minutes, and discard the supernatant.
6. Resuspend the cells in cell culture medium and examine a small aliquot under the microscope to assess the digestion process.
7. Use the cells for downstream experiments as needed.
8. (Optional) Red blood cell lysis buffer may be used to remove red blood cells or dead cells during tissue dissociation.
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Task: Share in the comments the famous quote that has most deeply resonated with you, and rally support by liking it.
• We’ll select the top 3 with the most likes and give away… Power bank 。
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Scope of Activities: National bio-related end customers
Precautions:
1. Follow the WeChat official account to participate in the event;
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3. The final interpretation of this event rests with Heyuan Liji.
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