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How do you perform a gradient dilution of serum?
Publication date：2026-10-29
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The replacement method is as follows: during the preparation of the complete culture medium, first mix 25% fresh serum with 75% original serum and perform 1–2 passages; then switch to a 50:50 mixture of fresh and original serum for subsequent passages; next, use a 75:25 ratio of fresh to original serum for further passages; and finally, continue all subsequent passages using only fresh serum.
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