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When thawing and culturing cells from cryovials, should DMSO be removed immediately?
Publication date:2026-10-29
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With the exception of a few cell lines specifically noted as sensitive to DMSO, most cell lines—including suspension‑type cells—can be directly transferred into 10–15 mL of fresh culture medium immediately after thawing; the medium can then be replaced with fresh medium the following day to remove the DMSO. This approach helps prevent the common issue of post‑thaw cell growth inhibition or poor attachment.
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