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What is the concentration of trypsin-EDTA used for passaging adherent cells, and how should it be handled?
Publication date：2026-10-29
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The commonly used concentration of trypsin-EDTA is 0.05% trypsin and 0.53 mM EDTA Na4. After opening the vial for the first time, aliquot small volumes into sterile tubes and store at −20 °C to prevent repeated freeze–thaw cycles that can reduce trypsin activity and minimize the risk of contamination.
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