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What are the methods for cryopreserving cells?
Publication date：2026-10-29
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Freezing preservation method 1: Place the cryovial in a 4°C incubator for 30–60 minutes, then transfer it to −20°C for 30 minutes*, followed by −80°C for 16–18 hours (or overnight), and finally store it in the vapor phase of liquid nitrogen for long-term preservation.
Freezing preservation method 2: Place the cryovial in a programmable freezer set to a controlled cooling rate of 1–3°C per minute until the temperature reaches below −80°C, then transfer it to the vapor phase of liquid nitrogen for long-term storage. *Note: Do not leave the vial at −20°C for more than 1 hour to prevent the formation of excessively large ice crystals, which can lead to significant cell death; alternatively, this step may be skipped and the vial directly placed in a −80°C freezer, though this will result in a slightly lower survival rate.
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