What kind of precipitate may appear in serum?
Publication date:2026-10-29
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Based on years of experimental research, the fetal bovine serum and other serums used for cell culture may contain the following types of precipitates:
(1) Fibrin: This is a common, relatively large precipitate, often measuring 1–2 mm in size and visible to the naked eye. Because serum is collected at low temperatures and processed rapidly, some fibrinogen—soluble precursors that form flocculent fibrin—remains dissolved during processing. After final filtration and aliquoting, it can coagulate in the vial, resulting in fibrin precipitation.
(2) Calcium phosphate: Another frequently observed precipitate, it typically causes turbidity in the serum and becomes more pronounced during incubation at 37°C. Under an inverted microscope, this precipitate appears as small black spots; due to Brownian motion, these spots may seem mobile, leading to frequent misidentification as microbial contamination.
(3) Cholesterol, fatty acid esters, and certain proteins: These are also common contributors to precipitate formation in serum.
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