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What should be kept in mind to ensure efficient cell cryopreservation?
Publication date：2026-10-29
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1. Slow freezing: Slow freezing allows cells to dehydrate gradually, preventing damage caused by the formation of large ice crystals. Conversely, the presence of large intracellular crystals can lead to damage and rupture of cell membranes and organelles.
2. Cell viability and concentration: Cells should be cryopreserved when they are in good growth condition, at a confluence of approximately 80–90% and with a viability exceeding 90%. Cells with poor viability exhibit very low post-thaw survival rates; therefore, it is essential to cryopreserve them during their active proliferative phase.
3. Cryopreservation density: If the cell density during cryopreservation falls below 5 × 10^5 viable cells/mL, successful recovery after thawing becomes difficult.
4. Cryovial caps: The caps of cryovials must be tightly sealed; otherwise, water may infiltrate during water-bath thawing, leading to contamination.
5. Storage duration: Cells should not be stored long-term at −80°C. We recommend limiting storage at −80°C to no more than 48 hours.
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