Product Center
Published literature
Technical Services
News and Information
Resource Center
About Life-ilab
返回
返回
返回
News and Information
What is the optimal time for cryopreserving cells?
Publication date：2026-10-29
Page views：
During in vitro cell culture, cells typically progress through three phases: the lag phase, the exponential growth phase, and the plateau phase. During the lag phase, which usually follows subculture, cells begin to adhere to the substrate and extend their cytoskeleton, with metabolic activity primarily focused on the expression of adhesion‑related proteins and cytoskeletal components; cell numbers remain virtually unchanged during this period.
Subsequently, cells enter the exponential growth phase, characterized by vigorous transcription and translation, frequent DNA unwinding and base pairing, and rapid intracellular transport, leading to a rapid increase in cell number. If cells are cryopreserved at this stage, they are effectively frozen at a specific point in their metabolic cycle, inevitably causing mechanical damage to unwound DNA as well as to RNA and proteins involved in transcription and translation, thereby increasing the risk of errors at various stages.
As cell numbers continue to grow, confluence in the culture vessel typically reaches 90% or higher. At this point, most cells cease rapid metabolism and proliferation due to contact inhibition, and intracellular activities gradually slow down.
Therefore, we recommend cryopreserving cells just as they enter the plateau phase, ensuring an adequate cell yield while minimizing mechanical stress on the cells.
Group Website
-
Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
WeChat Official Account
Online Customer Service 1
Online Customer Service 2
Telephone consultation
