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What are the main functions of serum in cell culture?
1. Provides hormones that maintain exponential cell growth, nutrients that are absent or present in very low amounts in the basal medium, and essential low-molecular-weight nutrients.
2. It provides binding proteins that can recognize vitamins, lipids, metals, and other hormones, and can bind to these molecules or modulate the activity of the substances they bind.
3. In some cases, proteins can bind to toxic metals and pyrogens, thereby exerting a detoxifying effect.
4. It is a source of factors required for cell adhesion and spreading on plastic culture substrates.
5. Acts as a pH buffer.
6. Provide a protease inhibitor to inactivate residual trypsin during cell passaging, thereby protecting the cells from damage.
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