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How many cells are typically required in a Western blot experiment?
Generally 5× 106 That’s sufficient. However, it depends on the cell type—some cells are very small, while others spread out quite extensively. In a standard Western blot, each lane typically loads a certain amount of sample… 105 A single cell. However, this also depends on the target protein and the extraction method. For membrane proteins or proteins localized within a specific organelle, the number of cells required will increase.
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