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When extracting RNA from pancreatic tissue, the sample consistently sticks to the mortar during grinding. How can this be addressed? Are there any effective methods?
After excising the pancreatic tissue, immediately immerse it in liquid nitrogen, then transfer it to a mortar (which has been dry‑heated at 180°C for 4–8 hours to inactivate RNases). Continue grinding, adding liquid nitrogen as you go, until a fine powder is obtained. At this stage, be sure to replenish the liquid nitrogen; if the tissue begins to thaw, it may adhere to the walls of the mortar. Once ground into a powder, add… Trizol At this point, since the low‑temperature Trizol will also freeze, continue grinding until the Trizol has completely melted and the mixture becomes clear and bright. Then centrifuge and proceed to the next steps. You can also use a homogenizer—it’s inexpensive and very easy to use—provided it has been treated to inactivate RNases. On ice, simply grind the tissue into a homogenate.
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