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After digesting adherent cells, can I centrifuge them and then add Trizol? Will this have any effect?
Publication date：2026-10-29
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Yes, you can resuspend the cells in a small volume of PBs and then extract RNA using Trizol; however, the extraction must be performed quickly and the sample should not be stored, as the cells are viable and their gene expression is influenced by the external environment. If the sample has already been stored for two hours, we recommend repeating the procedure.
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