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How can you determine whether cultured cells have become contaminated with bacteria?
After an overnight incubation of bacteria‑contaminated cells, the culture medium turns markedly yellow, and visible bacterial pellets can be seen in the flask. Agitation of the medium results in turbidity, and microscopic examination reveals fine, sand‑like particles covering the entire bottom of the flask. Differentiation between cellular debris and contamination can be made by observing the effect of an overnight incubation: if the medium remains clear, it is likely to be cellular debris or metabolic products; if it becomes noticeably yellow and turbid, contamination is confirmed. Bacterial contamination typically progresses within 1–2 days to a state visible to the naked eye. Cross‑contamination is uncommon, so promptly removing the contaminated cells is sufficient.
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