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What can be done to improve the situation when primary cells frequently become contaminated during isolation?
First, verify that the cell‑separation laboratory is free from bacterial contamination; if contamination is detected, disinfect the lab, sterilize the incubator, and confirm that all reagents and consumables are still viable. Second, strictly adhere to aseptic techniques to ensure that reagents, scissors, forceps, and other instruments remain sterile. Third, antibiotics such as penicillin–streptomycin or amphotericin B may be added to the culture medium.
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