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How do you control the digestion time of pancreatic enzymes?
Under an inverted microscope, cells become noticeably rounder and brighter, with increased intercellular spaces. Gently rocking the dish reveals suspended cells; stop when this stage is reached, as extensive detachment has not yet occurred. Avoid over‑digestion, which can compromise cell viability. For newly purchased cells, we recommend observing and recording the optimal timing every 30 seconds.
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