返回
返回
返回
Are trypsin and collagenase the same?
To dissociate cells from one another, enzymes can be used to digest the intercellular adhesion proteins, such as collagen, laminin, fibronectin, and elastin. The textbooks typically employ trypsin or collagenase; here, the use of “or” does not imply arbitrary selection, since these two enzymes target different substrates.
Trypsin is a pancreatic enzyme that efficiently digests soft tissues with sparse extracellular matrix, such as liver, kidney, thyroid, amnion, and embryonic tissues. However, it is ineffective against tissues rich in connective tissue, including mammary gland, synovium, uterus, fibrosarcoma, and tumor tissues. Moreover, prolonged digestion can damage cellular respiratory enzymes and cell membrane proteins, thereby harming the cells; therefore, cultured cells should be washed immediately after digestion.
Collagenase, an enzyme extracted from bacteria, is well suited for digesting fibrous tissues, epithelial tissues, and cancerous tissues. It exhibits strong proteolytic activity against collagen in the extracellular matrix; although its action is relatively slow, it has minimal impact on the cells themselves, thereby effectively achieving cell dissociation.
Group Website
-
Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
WeChat Official Account
Online Customer Service 1
Online Customer Service 2
Telephone consultation
