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What precautions should be taken when using pre-stained protein markers?
1) Due to the coupling between the pre-stained protein marker and the dye, the migration rates of the marker vary in different buffers; therefore, it is essential to select an appropriate buffer system before use. If an electrophoresis buffer not specified in the product instructions is employed, it is recommended to first calibrate the molecular weights of this pre-stained protein marker using a non‑prestained protein marker.
2) In low-concentration gels, low-molecular-weight proteins migrate ahead of the dye front.
3) For Western blotting of high-molecular-weight proteins, it is necessary to extend the transfer time or increase the transfer voltage.
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