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Are there other methods for detecting apoptosis?
Yes, the Annexin V assay is primarily used to detect early apoptosis; in the mid‑early stages, a decrease in mitochondrial membrane potential is often observed, which can be assessed using methods such as JC‑1 staining. In the mid‑late stages, activation of apoptotic signaling pathways occurs, and the level of Caspase‑3 activation is typically measured. In the late stage, DNA fragmentation within the nucleus becomes prominent, and this is commonly detected using the TUNEL assay.
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