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Can Annexin V apoptosis staining be performed on cells together with antibodies?

Publication date:2026-10-29
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Yes, but it is recommended to stain Annexin V separately from other antibodies. For example, when simultaneously labeling lymphocytes with Annexin V, PI, and CD3 or CD8, you can first incubate the cells in PBS to label CD3/CD8, then wash off the primary antibody, and finally stain with Annexin V in binding buffer (containing calcium ions).