Total disaster with cell transfection? We’re giving away a highly efficient, low-toxicity transfection reagent—free of charge!

Cell transfection It is a fundamental technique in cell biology that involves introducing exogenous molecules into (eukaryotic) cells to study gene function. Lipofection, one of the most widely used transfection methods, relies on transfection reagents whose critical role is often underestimated.

Have you ever encountered the classic scenario where experimental failure is caused by a substandard transfection reagent?

Scene One

After transfecting cells with a fluorescent plasmid, only weak fluorescence was observed even after 48 hours.

Possible causes: Weak fluorescence is generally indicative of low transfection efficiency. This may be due to substandard transfection reagents, or to poor quality and inappropriate concentration of the plasmid used, as well as excessively low cell density during transfection or poor cell health.

Solution: When selecting a transfection reagent, it is best to use a commercially available product that has been thoroughly validated. Under normal conditions, the plasmid concentration for cell transfection should exceed 500 ng/µL. Transfection should be performed during the logarithmic growth phase of the cells, and the optimal cell seeding density is typically around 90%–95%.

Scene Two

Twenty-four hours after transfection, observation revealed that the cells had already begun to die.

Possible causes: Cell death after transfection may result from the high toxicity of the transfection reagent, the addition of antibiotics during transfection, or failure to perform regular media changes following transfection.

Solution: Choose a transfection reagent with lower cytotoxicity, replace the antibiotic‑containing culture medium prior to transfection, and change the medium 4–6 hours after transfection.

To do a good job, one must first sharpen one’s tools! A high-quality transfection reagent can help us avoid many obstacles in our experiments.

Li Ji Biotechnology is supporting scientific research—starting today and running through May 31, 2024, you can enjoy a free trial of our transfection reagents!

▼EZ Trans Cell Transfection Reagent (High Efficiency)

As a next-generation transfection reagent, its transfection mechanism involves the formation of positively charged complexes between cationic polymers and the negatively charged phosphate groups of nucleic acids. These complexes then interact with negatively charged proteoglycans on the cell surface and are internalized into the cell via endocytosis.

It features high transfection efficiency, low cytotoxicity, simple operation, excellent reproducibility, and broad applicability.

▼EZ Trans Cell Transfection Reagent (Low Toxicity)

As a next-generation transfection reagent, its transfection mechanism involves the formation of positively charged complexes between cationic polymers and the negatively charged phosphate groups of nucleic acids. These complexes then interact with negatively charged proteoglycans on the cell surface and are internalized into the cell via endocytosis.

It features high transfection efficiency, low cytotoxicity, simple operation, excellent reproducibility, and broad applicability.

Compared with products such as Thermo, Lipo 3000, Invotrigen, and Lipofectamine 3000, it is more convenient to use.

▼EZ Trans Plus Cell Transfection Reagent (II)

As a next-generation transfection reagent, its transfection mechanism involves the formation of positively charged complexes between the cationic polymer and the negatively charged phosphate groups of nucleic acid molecules. These complexes then interact with the negatively charged proteoglycans on the cell surface and are internalized into the cell via endocytosis.

It features high transfection efficiency, low cytotoxicity, simple operation, excellent reproducibility, and broad applicability.

▼EZ Trans Lipo Cell Transfection Reagent (Lipid-Based)

EZ Trans Lipo encapsulates nucleic acids within nanoliposomes and, via a specialized delivery mechanism, facilitates the transfection of exogenous DNA, RNA, and siRNA into various cell types. It is suitable for transfecting both adherent and suspension cells and can be used in siRNA- and shRNA‑mediated gene knockdown experiments as well as gene expression studies.

It boasts high transfection efficiency, low cytotoxicity, broad cellular applicability, and excellent cost-effectiveness.

▼EZ Trans RNA Transfection Reagent

Using a novel liposomal nanomaterial, it is possible to transfect small RNA and DNA molecules—such as siRNA and miRNA—up to 150 base pairs in length. The cells that can be transfected include the vast majority of adherent cell types, such as common cell lines and tumor cell lines.

This transfection reagent is a rapid, efficient, ready-to-use solution. Simply mix the cell culture medium with the target small RNA—such as siRNA or miRNA—and the transfection reagent in an Eppendorf tube, incubate at room temperature for approximately 2 minutes, and then add the mixture to complete cell culture medium. Serum does not affect transfection efficiency, and no additional medium changes or additions are required during the experiment.