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2024-08-15Principle, experimental procedure, and precautions of the BCA method for protein quantificationProtein concentration determination is an essential method for calculating the recovery yield of purification procedures. Commonly used assays include the biuret method, the Lowry method, UV absorbance, and the BCA protein assay. Among these, the BCA method—also known as the bicinchoninic acid or bicinchoninate method—is the most widely employed.MORE -
2024-08-09How to deal with black mold contamination (black mold remover)Whether the black glue worm is a living organism or a non‑living entity remains a matter of debate in the scientific community; for now, it is provisionally regarded as a microorganism. The black glue worm typically originates from serum and is a common cellular contaminant that lives in symbiosis with cells, being passaged along with them during subculturing; antibiotics generally have no effect on it.MORE -
2024-08-09Principles and Procedures for Detecting Reactive Oxygen Species (ROS)Reactive oxygen species (ROS) are oxidatively active chemical compounds generated within cells, including hydrogen peroxide and the superoxide anion. ROS are produced under normal physiological conditions; however, when their production exceeds the cell’s capacity to detoxify them, oxidative stress ensues, leading to cellular damage and disease.MORE -
2024-08-08Qixi Festival Exclusive | Buy two pancreatic enzymes and dual‑antibody products, get one free—and enjoy exquisite gifts waiting for you!Full of love—can’t help but “enzyme” you!MORE -
2024-08-02Causes of Environmental Contamination in Laboratory Instruments and Solutions (Instrument‑Contamination Removal Reagents)In addition to contamination in cell cultures, contamination of laboratory equipment and the surrounding environment is also a major concern. This is particularly evident on incubator water trays, in incubators, in water baths, on cell culture workbenches, and in cell culture rooms.MORE -
2024-08-02Pre-stained Protein Marker: A Convenient and Efficient Tool for Protein Separation and IdentificationPre-stained protein markers, formally known as pre‑made protein molecular weight standards, are biochemical reagents used for protein electrophoresis and molecular weight determination.MORE -
2024-08-02Principles and Operational Procedures for PEI TransfectionPEI is a polycation with a high positive charge that readily binds to negatively charged DNA molecules, forming stable complexes. In cell lines such as HEK293 and CHO, PEI-based transfection yields high transfection efficiency and is particularly well suited for large-scale transient expression experiments.MORE -
2024-06-24Principle and Applications of the Dual-Luciferase Reporter Assay Kit (Dual-Luciferase Activity)Firefly luciferase is a protein with a molecular mass of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it catalyzes the oxidation of luciferin to oxyluciferin, emitting bioluminescence in the process.MORE -
2024-06-12Introduction to Tissue Dissociation Solutions and Instructions for UseTissue dissociation solution is a mixture of various biological enzymes that gently, rapidly, and efficiently enzymatically digest the extracellular matrix of tissues, releasing cells to form a cell suspension.MORE -
2024-06-12What is 1x PBS buffer (product advantages)?1X PBS (sterile) is a phosphate-buffered saline solution that is widely used in biological and biochemical research. It is commonly employed for washing tissue explants, rinsing cells, preparing other reagents, and serving as an alternative diluent for cell counting, among other applications.MORE
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