返回
返回
返回
Fetal Bovine Serum (FBS)
One click copying of product information
-
AC04L061300 µL800
-
AC04L0621 mL2200
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
-
Product Description
Serum contains proteins, amino acids, glucose, hormones, and other components; the proteins are primarily albumin and globulins. Amino acids are diverse and serve as the fundamental building blocks for cellular protein synthesis; some of these amino acids cannot be synthesized by animal cells themselves and are therefore classified as essential amino acids. The hormones present in serum include insulin, growth hormone, and various growth factors, such as epidermal growth factor, fibroblast growth factor, and insulin-like growth factors. Additionally, serum harbors numerous unidentified cell‑growth‑promoting factors, adhesion‑enhancing factors, and other bioactive substances that stimulate cell growth, proliferation, and attachment.
At Li Ji Bio, each batch of fetal bovine serum undergoes rigorous screening of the source blood material and is subjected to three consecutive filtration steps through 0.1 μm membrane filters, with filter materials compliant with U.S. FDA requirements. The entire manufacturing process adheres to international standards for fetal bovine serum production and is subject to stringent quality control, including comprehensive physicochemical and microbiological testing. Key parameters such as pH, osmolality, hemoglobin concentration, endotoxin levels, and total protein all meet established specifications, with no detectable contamination by bacteria, fungi, mycoplasma, or viruses. Batch-to-batch consistency ensures suitability for a wide range of cell culture applications, including primary cells and certain stem cell lines.
Order Information
Product Name
Item number
Specifications
Fetal Bovine Serum (FBS)
AC03L155
500 mL
Fetal Bovine Serum (FBS)
AC03L156
10*50 mL
Transportation and Storage
Dry ice transportation. Store at 4°C for short-term use; store at −15°C to −40°C with a shelf life of 60 months.
Precautions
1. This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
2. Serum Thawing and Storage: After removing the serum from a −20°C or lower freezer, it is recommended to thaw it in a 4°C refrigerator for approximately [duration not specified], gently shaking it at intervals to ensure uniformity. Do not place the serum directly in a warmer environment to avoid rapid temperature changes that may cause protein aggregation and precipitation, thereby compromising serum quality. If a vial cannot be used up within a short period, it is advisable to aseptically aliquot the remaining portion and return it to freezing to prevent repeated freeze–thaw cycles. Prolonged storage at 4°C may lead to the formation of denatured lipoproteins and fibrin, resulting in flocculent precipitates and a decline in serum quality.
3. Serum inactivation: Heating can inactivate the complement system in serum. In very rare cases—such as when culturing ES cells or smooth muscle cells—inactivation is recommended; for all other cell types, inactivated serum is not advised. Serum inactivation readily leads to precipitation; if inactivation is necessary, follow the protocol of 56°C for 30 minutes with gentle shaking. Higher temperatures, longer incubation times, or uneven shaking can all increase the likelihood of precipitate formation.
4. Serum-free media: Serum-free media offer advantages in terms of assay reproducibility and in vitro cell differentiation; however, serum remains the most essential component of culture media, particularly in primary cultures or when cells exhibit poor growth. In such cases, serum-containing medium is often used initially to promote robust cell growth, with serum-free medium substituted once the cells have reached a vigorous growth phase.
Related Product Recommendations
EZ Trans Cell Transfection Reagent (High Efficiency) (Catalog No.: AC04L092)
Rapid Cell Cryopreservation Solution (Serum-Free, Protein-Free) (Catalog No.: AC05L033)
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
-
Q: How does Li Ji Bio ensure the quality of its premium fetal bovine serum (FBS) products?
A: Li Ji Bio’s FBS undergoes rigorous batch‑by‑batch screening, requiring three consecutive 0.1 µm membrane filtration steps; the filtration materials comply with U.S. FDA documentation requirements. The entire production process adheres to international standards for fetal bovine serum manufacturing and is subject to stringent quality control, including comprehensive physicochemical and microbiological testing.
Q: How long can fetal bovine serum be stored at 4°C?
A: Fetal bovine serum can typically be stored at 4°C for 6 to 12 months. However, this is not an absolute guideline; the actual shelf life depends on various factors, such as the serum’s quality, the airtightness of the container, and the stability of the freezer. To ensure optimal quality, it is recommended to use the serum within three months of purchase. For long-term storage, freezing at –20°C or lower is ideal, allowing it to remain stable for one to two years or even longer. Note that each time you aliquot the serum, avoid repeated freeze–thaw cycles to prevent loss of proteins and nutrients. Before use, carefully read the product instructions and follow the manufacturer’s recommendations.
Q: Sometimes flocculent precipitates appear in the serum. What causes this, and can the product still be used?
A: ① When stored at 2–8°C, fibrin, lipoproteins, cold agglutinins, fibronectin, and other components potentially present in the serum may aggregate, forming visible precipitates or turbidity. This typically does not affect the serum’s performance. If precipitation needs to be removed, centrifuge at 400×g for 5 minutes, then use the supernatant. ② Common causes of such precipitation include abrupt temperature changes (e.g., thawing directly from −20°C to 37°C), repeated freeze–thaw cycles, heat inactivation (treatment at 56°C), and prolonged storage at 4°C or room temperature. To minimize precipitation, it is recommended to thaw slowly at 4°C while gently mixing, aliquot and store to avoid repeated freeze–thaw cycles, and, whenever possible, refrain from heat inactivating the serum, as this can lead to increased or new precipitation.
Q: Does the serum need to be heat‑inactivated?
A: Depending on the cell source or type, most insect cell lines and embryonic stem cell lines require heat‑inactivated serum. If heat inactivation is necessary, we recommend a 30‑minute incubation at 56°C in a water bath, with gentle shaking to ensure uniformity. This method helps preserve the serum’s nutritional components while effectively removing complement.
Q: For certain sensitive cell types, such as primary neurons, what precautions should be taken when changing the serum and trypsin?
A: Taking primary neuronal cells as an example, they are typically quite sensitive. When replacing the culture medium and serum, it is recommended to use a stepwise replacement approach—for instance, starting with a mixture of 25% fresh serum and 75% old serum, gradually increasing the proportion of fresh serum. When switching the digestive enzyme (e.g., trypsin), the enzyme concentration should be appropriately reduced to minimize cellular stress and damage. Additionally, depending on the specific cell type and experimental requirements, it may be necessary to supplement with particular nutrients or growth factors. It is advisable to consult relevant literature to determine the most suitable supplementation strategy.
Q: How does selecting an appropriate serum affect the culture of primary cells?
A: When culturing primary cells, it is important to account for the heterogeneity in proliferative capacity within the cell population. The closer the culture conditions are to the in vivo microenvironment, the more favorable they will be; cells with strong proliferative potential—such as progenitor cells—are more likely to dominate, thereby increasing the number of passages. Conversely, if terminally differentiated cells predominate, the cells’ ability to be passaged will be limited.
-
Fill in the product batch number for inquiry
-
Fill in the product batch number for inquiry
Best Sellers
Popular Articles
Group Website
-
Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
WeChat Official Account
Online Customer Service 1
Online Customer Service 2
Telephone consultation
