EZ ECL pico chemiluminescent solution (ultrasensitive)
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AP34L024100mL198
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AP34L025500 mL898
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- MSDS
- Operation video
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Product Description
EZ ECL pico chemiluminescent solution is a highly sensitive, enhanced chemiluminescence (ECL) HRP substrate that enables users to detect proteins at sub-picongram levels (<1×10⁻¹² g) in Western blotting assays. -12 g) Extremely antibody‑efficient. The EZ ECL pico substrate delivers bright signals and picogram‑level detection sensitivity in Western blotting assays using horseradish peroxidase (HRP)-conjugated antibodies. This ECL substrate is compatible with a wide range of membranes, blocking solutions, and antibody diluents, offering superior performance, broad applicability, and excellent cost‑effectiveness to meet the needs of users’ Western blotting applications.
Characteristics of the EZ Pico substrate:
High sensitivity: detects picogram‑level protein bands on nitrocellulose or PVDF membranes.
Long signal duration: Under optimized conditions, the blotting strip incubated with the substrate can sustain a detectable luminescent signal for 6 to 8 hours.
Recommended dilution concentration:
Primary antibody concentration: It is recommended to start with a 1 mg/mL stock solution at a dilution of 1:1,000–1:5,000, or 0.2–1 µg/mL.
Secondary antibody concentration: It is recommended to start with a 1 mg/mL stock solution diluted to 1:20,000–1:100,000, or to a final concentration of 10–50 ng/mL.
Order Information
Product Name
Item number
Specifications
EZ ECL Pico Chemiluminescent Solution (Ultra-Sensitive)
AP34L024
100 mL
EZ ECL Pico Chemiluminescent Solution (Ultra-Sensitive)
AP34L025
500 mL
Product ingredients
Component
AP34L024
AP34L025
Ultra-sensitive Type A solution
50 mL
250 mL
Ultra-sensitive Type B liquid
50 mL
250 mL
Transportation and Storage
Blue ice transport. Store at 4°C away from light; shelf life: 24 months.
ECL Chemiluminescent Reagent Operation Demonstration Video
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Fill in the product batch number for inquiry
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Fill in the product batch number for inquiry
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ECL Chemiluminescent Reagent Operation Demonstration Video
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