返回
返回
返回
EZ Lentiviral Infection Enhancer
One click copying of product information
-
AC04L443250 µL680
-
AC04L444500uL1180
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
-
Product Description
EZ Lentiviral Infection Enhancer (hereinafter referred to as “EZ Enhancer”) can significantly boost the efficiency of retroviral infection in target cells. Through formulation optimization, this product further enhances the binding between cell-surface sialic acids and viral particles, greatly increasing the efficiency of viral entry into cells. Following optimization, the reagent exhibits reduced side effects, more consistent dosing, and broader applicability across a wider range of cell types.
Significant effect: It can significantly enhance the efficiency of lentiviral infection, effectively reducing the amount of virus required.
Highly adaptable: It exhibits virtually no cytotoxicity and is compatible with sensitive cells.
Good stability: The product is stable and has a long shelf life.
Product ingredients
Product Name
Item number
Specifications
EZ Lentiviral Infection Enhancer
AC04L443
250uL
EZ Lentiviral Infection Enhancer
AC04L444
500uL
Transportation and Save
Blue ice transport. Store at −20°C; shelf life: 12 months.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
-
Q: What precautions should be taken after infecting adherent cells?
A: After infecting cells with the virus for 72 hours, if the vector carries a fluorescent protein (such as GFP or mCherry), you can observe the cells under a fluorescence microscope to assess the efficiency of lentiviral infection in the target cells. If the fluorescence is weak, extend the observation period to 96 hours. Select an appropriate time point for your subsequent experiments based on their specific requirements. If the vector does not contain a fluorescent protein, you cannot directly evaluate viral infection efficiency by fluorescence microscopy; instead, you can use qPCR, Western blotting, immunofluorescence with the target antibody, or flow cytometry with the target antibody to assess viral expression levels.
Q: What precautions should be taken after infecting suspension cells?
A: After infecting cells with the virus for 72 hours, observe the cells under a fluorescence microscope to assess the efficiency of lentiviral transduction into the target cells. If fluorescence is weak, extend the observation period to 96 hours. Based on the specific requirements of the subsequent experiments, select an appropriate time point for proceeding.
Q: What precautions should be taken after infecting primary neurons?
A: After infecting cells with the virus for 72 hours, observe the cells under a fluorescence microscope to assess the infection efficiency. If fluorescence is weak, extend the observation period to 96 hours. Select an appropriate time point for subsequent experiments based on the specific experimental design.
Q: What are the advantages of Li Ji’s product compared to domestic Brand B?
A: The novel experimental reagent Polybrene‑pus virus infection enhancement solution is a cationic polymer that enhances cellular permeability by directly interacting with the cell membrane, thereby facilitating viral particle entry and improving lentiviral transduction efficiency. Its performance in enhancing lentiviral infection is significantly superior to that of polybrene. This product can substantially increase cell infection rates; unlike polybrene, it exhibits no cytotoxicity and eliminates the need to change the culture medium the day after infection. When conventional culture media yield poor transduction results, this solution can be added to the culture medium at a final working concentration of 1 µg/µL. Advantages: It boosts viral infection efficiency, reducing the amount of virus required and lowering costs. The product is stable and has a long shelf life.
-
Fill in the product batch number for inquiry
-
Fill in the product batch number for inquiry
Best Sellers
Popular Articles
Group Website
-
Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
WeChat Official Account
Online Customer Service 1
Online Customer Service 2
Telephone consultation
