“He Yuan Li Ji” was established as a joint venture between He Yuan Bio (stock code: 688238) and Li Ji Bio, with its core business centered on the “Li Ji Bio” and “Life-ilab” reagent brands.

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Quick Cell Mycoplasma Rapid Detection Kit (for Cell Culture Use Only)

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Product Item Number
Product Specifications
Original price
  • AC16L061
    50T
    1580
  • Product Description
  • Product FAQ
  • COA
  • Product Literature
  • MSDS
  • Operation video
  • Product Description

    Quick Cell The Mycoplasma Rapid Detection Kit (for Cell Culture Use Only) is specifically designed to quickly detect the presence of mycoplasma contamination in cell culture supernatants or other liquid samples, such as serum. This kit is easy to use and features a short reaction time: simply add 1 μL of cell culture supernatant to the reaction mixture, incubate at 60°C for 1 hour, and interpret the results by visual inspection. It offers clear advantages over traditional PCR‑based methods for mycoplasma detection. The kit has a broad detection range, capable of identifying: (1) M. hyorhinis, (2) M. fermentans, (3) M. arginini, (4) M. hominis, (5) M. oralis, (6) M. salivarium, (7) M. pirum, (8) Acholeplasma laidlawii, (9) M. agalactiae, (10) M. bovis, (11) M. buccale, (12) M. arthritidis, and (13) M. pulmonis—nearly all common mycoplasmas that contaminate cell cultures (where “M.” stands for Mycoplasma). These 13 species account for more than 99% of mycoplasma contaminations in cell cultures, with the vast majority concentrated among the first eight. Consequently, this kit is ideally suited for routine mycoplasma testing in research laboratories and enterprises engaged in cell biology. [Note]: The rapid method cannot detect Ureaplasma or Mycoplasma pneumoniae.

    Order Information

    Product Name

    Item number

    Specifications

    Quick Cell Mycoplasma Rapid Detection Kit (for Cell Culture Use Only)

    AC16L061

    50 T

    Product ingredients

    Component

    Specifications

    A.              Solution I

    1075 μL (50 tests)

    B.             Solution II

    55 μL (50 tests)

    C.             Solution III

    Indicator, ≥38 μL (50 tests)

    D.             Positive Mycoplasma DNA

    50 microliter

    E.              Mineral oil

    1500 microliter

    [Note]: ① The “50 tests” indicated for this kit include both negative and positive controls; it does not refer to 50 individual samples. Since a control is required for each run, the number of samples tested will depend on the experimental design. In theory, up to 48 samples can be processed in a single run. ② Prior to use, gently centrifuge the sample tubes to prevent reagent loss due to adherence to the tube walls, which could reduce the number of detectable samples. (Note: Positive Mycoplasma DNA samples do not require centrifugation.)

    Transportation and Storage

    Blue‑ice transport. Store at −20°C; shelf life: 36 months. [Note]: Solution III is more stable when stored at −80°C.

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    This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.

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