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Quick Cell PCR Mycoplasma Detection Kit
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AC16L064100T1680
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Product Description
Quick Cell PCR Mycoplasma Detection Kit. This kit employs a pair of mycoplasma-specific primers to amplify the genomic DNA of mycoplasmas in the sample, followed by detection of the PCR products via agarose gel electrophoresis. The kit also includes an internal control to verify that the PCR amplification is proceeding correctly.
This kit is a broad‑spectrum mycoplasma detection assay capable of identifying all more than 100 known mycoplasma species. It can be used to test any sample that may harbor mycoplasma, including supernatants from in vitro cell cultures, serum, various bodily fluids such as saliva, urine, and nasal secretions, as well as other liquid samples. Key advantages include 100% mycoplasma detection specificity, extremely high sensitivity, the inclusion of an internal control band to distinguish true‑negative from false‑negative results, and the fact that it does not require a costly real‑time quantitative PCR instrument.
After extensive testing, this kit has been shown to detect the 20 mycoplasma species previously reported in in vitro cell cultures; according to the literature, these species account for nearly 100% of the mycoplasma contaminating cultured cells. Specifically: (1) M. hyorhinis, (2) M. fermentans, (3) M. arginini, (4) M. hominis, (5) M. orale, (6) M. salivarium, (7) M. pirum, (8) A. laidlawii, (9) M. agalactiae, (10) M. bovis, (11) M. bovoculi, (12) A. axanthum, (13) M. buccale, (14) M. pneumoniae, (15) M. arthritidis, (16) M. pulmonis, (17) M. gallisepticum, (18) M. gallinarum, (19) M. canis, and (20) Ureaplasma urealyticum. (Note: “M.” stands for Mycoplasma; “A.” stands for Acholeplasma.)
Product ingredients
Product Component
Specifications
Mycoplasma primers and internal control (100 reactions)
206 μL
Positive Mycoplasma DNA
50 μL
Deionized water
1.8 mL
(Please use ordinary distilled water or deionized water; do not use endotoxin‑free water.)
[Note] : The following reagents must be prepared independently: ① Standard Taq DNA polymerase (Takara’s Ex Taq Hot Start version, catalog number RR006A, which already contains 2.5 mM dNTPs) and the corresponding buffer; ② 2.5 mM dNTPs; ③ DNA loading buffer (Li Ji Bio’s is recommended). GelRed Pre-stained DNA Loading Buffer (5X), Catalog No.: AN34L047 , this buffer already contains a non‑toxic nucleic acid dye, so there is no need to handle carcinogenic substances such as EB.
Transportation and Storage
Blue ice transport. Store at −20°C; shelf life: 60 months.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
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