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EdU (animal injection)
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AC11L2712mg680
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AC11L27210mg1580
- Product Description
- Product FAQ
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- MSDS
- Operation video
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Product Description
Cell proliferation is an important indicator for assessing cellular viability, metabolism, and physiological and pathological conditions. EdU (5-ethynyl-2′-deoxyuridine) is a thymidine analog whose terminal alkyne group is rarely found in natural compounds. During cell proliferation, EdU can substitute for thymine (T) and become incorporated into replicating DNA molecules. By exploiting the specific conjugation reaction between EdU and fluorescent dyes, fluorescent labels can be attached to newly synthesized DNA containing EdU, enabling efficient and rapid detection of DNA replication activity. This technique is widely used in research on cell proliferation, cell differentiation, growth and development, DNA repair, and other related processes.
Traditional immunofluorescence staining methods using BrdU require complex and time‑consuming procedures, such as DNA denaturation, antigen retrieval, and overnight incubation of antibodies with antigens. In contrast, the EdU assay does not necessitate harsh DNA denaturation; it only requires mild cell fixation and permeabilization, which better preserve cellular morphology, the overall DNA structure, and intracellular antigen‑binding sites. This results in a faster, more sensitive, and more accurate workflow. EdU closely resembles thymidine (T) in structure yet is only about 1/500 the size of the BrdU antibody, allowing it to diffuse readily within cells. Moreover, because it bypasses the need for antigen–antibody binding, EdU can more rapidly and conveniently report DNA replication activity at both the cellular and tissue levels.
This reagent is suitable for assessing cellular proliferation in various tissues and organs of mouse, rat, and other animal models.
Product ingredients
Product ingredients
Reagent amount
Specifications
EdU powder
2 mg
20 T
EdU powder
10 mg
100 T
Transportation and Storage
Store at room temperature. Keep refrigerated at 4°C; shelf life is 12 months.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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Q: Using a long-term labeling period (e.g., 7 days or longer, such as in the brain). Does this long-term labeling mean that after a single injection, we wait for 7 days, or do we inject daily for 7 days?
A: Long-term labeling means administering a single injection and waiting for 7 days.
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