Annexin V-647/PI Cell Apoptosis Assay Kit
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AC12L04250T880
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AC12L043100T1580
- Product Description
- Product FAQ
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- MSDS
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Product Description
The Annexin V‑647/PI apoptosis assay kit provides a rapid and convenient method for assessing apoptotic levels by labeling early apoptotic cells (far red) and necrotic cells (red). The assay can be performed using a flow cytometer or other fluorescence‑based detection instruments.
Annexin V‑647 can label apoptotic cells. Annexin V selectively binds to phosphatidylserine (PS). During the early stages of apoptosis, PS translocates to the cell surface, exposing its outer leaflet. Annexin V labeled with the far-red fluorescent dye 647—Annexin V‑647—can bind to this externalized phosphatidylserine, thereby detecting a key hallmark of apoptosis. Compared with conventional fluoresceins, Li Ji Bio’s 647 dye exhibits higher fluorescence intensity, is insensitive to environmental pH, and demonstrates excellent photostability.
Propidium Iodide (PI) is a DNA-binding dye that stains the nuclei of necrotic cells or cells in the late stages of apoptosis, which have lost plasma membrane integrity. PI can be excited by lasers at 488, 532, or 546 nm and emits red fluorescence.
Order Information
Product Name
Item number
Specifications
Annexin V-647/PI Cell Apoptosis Assay Kit
AC12L042
50T
Annexin V-647/PI Cell Apoptosis Assay Kit
AC12L043
100T
Product ingredients
Component
AC12L042( 50T)
AC12L043( 100T)
A. 1× Annexin V Binding Buffer
50 mL
2* 50 mL
B. Annexin V-647
250 μL
500 μL
C. PI
500 μL
1 mL
Transportation and Storage
Blue ice transport. Store at 4°C away from light; shelf life: 24 months.
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This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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