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One-Step 594 TUNEL Cell Apoptosis Assay Kit (Red)
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AC12L05220T1180
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AC12L05350T2280
- Product Description
- Product FAQ
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- Product Literature
- MSDS
- Operation video
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Product Description
When cells undergo apoptosis, certain DNA endonucleases are activated; these endonucleases cleave genomic DNA between nucleosomes, generating A DNA fragment of 180–200 bp exhibits a characteristic ladder‑like pattern on agarose gel electrophoresis. Double‑stranded genomic DNA or… When a single-strand break occurs, numerous sticky 3′-OH termini are generated, which, under the catalytic action of terminal deoxynucleotidyl transferase (TdT), can be linked to… dUTP incorporation, thereby enabling direct detection of apoptotic cells by fluorescence microscopy or flow cytometry; this approach is referred to as deoxyribonucleotide end labeling. Terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL), TUNEL). Because normal or proliferating cells exhibit virtually no DNA fragmentation, they lack 3′-OH groups and are rarely stained. The TUNEL assay enables in situ staining of intact individual apoptotic nuclei or apoptotic bodies, accurately reflecting the typical biochemical and Morphological characteristics allow for the detection of even very few apoptotic cells, making this method widely used in studies of cellular apoptosis.
This kit has a broad range of applications and can be used to detect apoptosis in frozen or paraffin‑embedded tissue sections, as well as in cultured adherent cells or suspension cultures. Apoptotic status of floating cells. It allows for the selective detection of apoptotic cells, as opposed to necrotic cells or DNA strand breaks induced by irradiation and pharmacological treatment. Cells. This kit detects cellular apoptosis with a short turnaround time, requiring only a single staining step; detection can be performed immediately after washing.
Order Information
Product Name
Item number
Specifications
One-Step 594 TUNEL Cell Apoptosis Assay Kit (Red)
AC12L052
20T
One-Step 594 TUNEL Cell Apoptosis Assay Kit (Red)
AC12L053
50T
Product ingredients
Component
20 T
50 T
A.EZ 594 TUNEL Reaction Buffer
1 mL
2 × 1.25 mL
B.TdT Enzyme
40 μL
100 μL
C.Proteinase K (2 mg/mL)
40 μL
100 μL
D.DNase I (2 U/μL)
5 μL
13 μL
E.10 × DNase I Buffer
100 μL
260 μL
Transportation and Save
Blue ice transport. Store at −20°C; shelf life: 24 months. Note: Component A should be stored away from light and protected from repeated freeze-thaw cycles.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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