返回
返回
返回
JC-1 Mitochondrial Membrane Potential Assay Kit
One click copying of product information
-
AC12L07220T580
-
AC12L073100T1180
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
-
Product Description
A decrease in mitochondrial membrane potential (ΔΨm) is a key hallmark of early apoptosis. This decline in membrane potential arises from alterations in mitochondrial membrane permeability, primarily driven by pro-apoptotic proteins—such as the formation of Bax dimers and the activation of Bid, Bak, and Bad—which induce the opening of pores in the outer mitochondrial membrane. Concurrent with the activation of these proteins, cytochrome c is released from the mitochondria into the cytoplasm.
JC‑1 is an ideal fluorescent probe widely used for detecting ΔΨm, suitable for cells, tissues, or purified mitochondrial samples. Its detection principle relies on a membrane potential–dependent fluorescence state transition: when the mitochondrial membrane potential is high, JC‑1 aggregates in the mitochondrial matrix to form polymers, emitting red fluorescence; when the mitochondrial membrane potential is low, JC‑1 fails to aggregate in the matrix and exists as monomers, yielding green fluorescence. The shift from red to green fluorescence upon JC‑1 aggregation provides a straightforward readout of membrane potential decline and can also serve as an early biomarker of apoptosis.
The JC‑1 monomer has a maximum excitation wavelength of 510 nm and a maximum emission wavelength of 527 nm; the JC‑1 aggregate exhibits a maximum excitation wavelength of 585 nm and a maximum emission wavelength of 590 nm. This kit is easy and rapid to use, and can be detected using a flow cytometer, a fluorescence microscope, or a fluorescence microplate reader.
Order Information
Product Name
Item number
Specifications
JC-1 Mitochondrial Membrane Potential Assay Kit
AC12L072
20T
JC-1 Mitochondrial Membrane Potential Assay Kit
AC12L073
100T
Product ingredients
Component
Specifications ( 20T )
Specifications ( 100T )
A. JC-1, 100× in DMSO
100 microliter
500 microliter
B. 10× Assay Buffer
5mL
25mL
C. CCCP, 50 mM
10 microliter
50 microliter
Transportation and Save
Blue ice transport. Store at –20°C away from light; shelf life: 36 months. Note: Component B may also be stored at 4°C. To prevent repeated freeze–thaw cycles, it is recommended to aliquot Components A and C.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
-
Fill in the product batch number for inquiry
-
Fill in the product batch number for inquiry
Best Sellers
Popular Articles
Group Website
-
Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
WeChat Official Account
Online Customer Service 1
Online Customer Service 2
Telephone consultation
