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Collagenase, Type III
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AC15L242100mg450
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Product Description
Collagenase is a crude enzyme preparation derived from Clostridium histolyticum, also known as clostridiopeptidase A. It contains not only collagenase but also a mixture of several other enzymes that work together to dissociate cells and tissues. This “crude” collagenase preferentially degrades the helical regions of native collagen and can cleave multiple times within the triple‑helix structure, targeting the Pro‑Y‑Gly‑Pro sequence—common in collagen and the principal fibrous component of extracellular connective tissue in animals—at the Y‑Gly bond, where Y is most often a neutral amino acid. Compared with trypsin, collagenase exhibits milder digestion, maintaining excellent dissociation activity under physiological temperature and pH conditions without the need for mechanical agitation.
Currently, commercially available bacterial collagenases are classified into four types based on differences in collagenase activity: Type I, Type II, Type III, and Type IV, each with specific applications: (1) Type I Collagenase contains a relatively balanced spectrum of enzymatic activities, including collagenase, caseinase, clostridial protease, and trypsin. It is commonly used for the isolation of epithelial cells, as well as cells from liver, lung, adipose tissue, and adrenal gland tissues. (2) Type II Collagenase exhibits higher clostridial protease activity and is typically employed for isolating cells derived from heart, bone, muscle, thymus, and cartilage tissues. (3) Type III Collagenase has lower proteolytic activity and is frequently used for the preparation of mammary gland cells. (4) Type IV Collagenase features low trypsin activity and is generally utilized for isolating pancreatic islet cells or for experiments requiring the preservation of membrane proteins or receptors.
Product ingredients
Product Name
Item number
Specifications
Collagenase, Type III
AC15L242
100 mg
Transportation and Save
Blue ice transport; store at 4°C. Shelf life: 24 months.
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This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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