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Biotin TUNEL Assay Apoptosis Detection Kit
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AC12L08220T1180
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AC12L08350T2280
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- Product FAQ
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- Product Literature
- MSDS
- Operation video
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Product Description
A hallmark of apoptosis is the degradation of cellular chromosomal DNA, a phenomenon that is quite widespread. This degradation is highly specific and orderly, yielding DNA fragments of lengths that are integer multiples of approximately 180–200 base pairs, which manifest as a characteristic ladder‑like pattern on agarose gel electrophoresis. This kit employs the TUNEL assay, in which terminal deoxynucleotidyl transferase (TdT) catalyzes the incorporation of biotin‑labeled dUTP (Biotin‑X‑dUTP) at the 3′‑OH ends of fragmented DNA in apoptotic cells. The incorporated biotinylated nucleotides then specifically bind to streptavidin conjugated with horseradish peroxidase (Streptavidin‑HRP). Finally, under HRP catalysis, DAB staining visualizes apoptotic cells, allowing their detection and enumeration by conventional light microscopy. Because normal or proliferating cells exhibit minimal DNA fragmentation and thus lack 3′‑OH groups, they are rarely stained. The TUNEL assay enables selective, direct in situ detection of apoptotic cells, distinguishing them from necrotic cells or those undergoing DNA strand breaks induced by irradiation or pharmacological treatments, making it a faster and more straightforward method for assessing apoptosis.
Order Information
Product Name
Item number
Specifications
Biotin TUNEL Assay Apoptosis Detection Kit
AC12L082
20T
Biotin TUNEL Assay Apoptosis Detection Kit
AC12L083
50T
Product ingredients
Component
AC12L082 (20T)
AC12L083 (50T)
A. Biotin TUNEL Reaction Buffer
2*0.5 mL
5*0.5 mL
B. TdT enzyme
20 μL
50 μL
C. Streptavidin-HRP
20 μL
50 μL
D. Streptavidin-HRP Diluent
1 mL
2*1.25 mL
E. DAB chromogenic solution A
100 μL
250 μL
F. DAB Chromogen Solution B
1 mL
2.5 mL
G. DAB chromogenic solution C
50 μL
125 μL
H. Proteinase K (2 mg/mL)
40 μL
100 microliter
I. DNase I (2 U/μL)
5 μL
13 μL
J. 10 × DNase I Buffer
100 μL
260 μL
Transportation and Save
Blue Ice Shipping. Store at −20°C away from light; Components A, E, and G must be protected from light and avoid repeated freeze–thaw cycles. Shelf life is indicated on the outer packaging. [Note]: When using components A, E, F, and G, please wear a mask and gloves. If the product comes into contact with skin, rinse immediately with plenty of water.
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This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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