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0.25% trypsin digestion solution (containing EDTA, containing phenol red)
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AC15L811100mL50
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Product Description
Trypsin, often referred to as pancreatic enzyme, is a serine protease that cleaves the carboxyl‑terminal bonds of lysine and arginine residues in polypeptide chains. During the isolation and culture of tissue cells, as well as in in vitro cell culture, trypsin digestion solutions are routinely used to hydrolyze intercellular proteins, thereby dissociating tissues or cell aggregates into single cells.
Pancreatic enzymes exhibit their highest digestive activity at 37°C and pH 8.0. A commonly used working concentration of pancreatic enzyme is 0.25%. EDTA chelates calcium and magnesium ions; when added to a pancreatic enzyme solution, it accelerates the hydrolysis of intercellular junction proteins, thereby enhancing digestion. Phenol red is a pH indicator: it appears purplish‑red in alkaline solutions, orange‑red in acidic solutions, and pink near neutral pH.
This product contains 0.25% trypsin, is dissolved in Hank’s buffer, supplemented with 0.9 mM EDTA, and includes a phenol red indicator; it has been sterilized by filtration through a 0.22 μm membrane.
Product ingredients
Product Name
Specifications
0.25% trypsin digestion solution (containing EDTA, containing phenol red)
100 mL
Transportation and Storage
Blue ice transport. Store at −20°C; shelf life: 24 months. [Note]: Avoid repeated freeze-thaw cycles.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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Q: How long can it be stored at 4°C?
A: For the first use, thaw at 4°C, aliquot into small vials, and store at −20°C. For short-term use, keep at 4°C and use within two weeks; do not exceed one month.
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