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0.25% trypsin digestion solution (containing EDTA, phenol red–free)
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AC15L821100mL
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Product Description
Trypsin, often referred to simply as pancreatic enzyme, is a serine protease that cleaves peptide bonds at the carboxyl side of lysine and arginine residues. During tissue cell isolation and culture, as well as in vitro cell culture, trypsin digestion solutions are routinely used to hydrolyze intercellular proteins, thereby dissociating tissues or cells into single cells. Trypsin exhibits its highest enzymatic activity at 37°C and pH 8.0. A commonly used working concentration of trypsin is 0.25%. EDTA chelates calcium and magnesium ions; when combined with trypsin, it accelerates the hydrolysis of intercellular adhesion proteins, enhancing the digestion process. Phenol red is a pH indicator: it appears purplish‑red under alkaline conditions, orange‑red under acidic conditions, and pink near neutral pH.
This product contains 0.25% trypsin, is dissolved in Hank’s buffer, includes 0.9 mM EDTA, and is free of phenol red indicator; it has been sterilized by filtration through a 0.22 μm membrane.
Product ingredients
Product Name
Specifications
0.25% trypsin digestion solution (containing EDTA, phenol red–free)
100 mL
Transportation and Storage
Blue ice transport. Store at −20°C; shelf life: 24 months. 【 Note 】: Avoid repeated freeze-thaw cycles.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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Fill in the product batch number for inquiry
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Q: How long can it be stored at 4°C?
A: For the first use, thaw at 4°C, aliquot into small vials, and store at −20°C. For short-term use, keep at 4°C and use within two weeks; do not exceed one month.
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