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Smart Cell Ceramide Golgi Staining Kit, Green
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AC14L313100 tests2980
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
The Golgi apparatus is a network of vesicles and folded membranes in the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport. It modifies proteins and lipids synthesized in the endoplasmic reticulum (ER) and prepares them for export outside the cell. It also plays a crucial role in the intracellular trafficking of lipids and in the formation of lysosomes.
The Smart Cell NBD Ceramide Golgi Staining Kit provides a simple, rapid method for selectively staining the Golgi apparatus in live or aldehyde‑fixed cells. Cells are incubated with C6 NBD ceramide complexed with bovine serum albumin (C6‑NBD‑Ceramide‑BSA). The Golgi apparatus is stained through the formation of corresponding fluorescent metabolites.
Product ingredients
Component
Quantity
Component A: C6 NBD Ceramide
1 vial
Component B: Staining Buffer
1 bottle (25 mL)
Component C: DMSO
1 vial (200μL)
Component D: Hoechst 33342
1 vial (50μL)
Transportation and Storage
Blue ice transport. Store at -20°C away from light; shelf life: 12 months.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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Q: What are the excitation and emission wavelengths of this product?
A: Green fluorescence: Excitation wavelength: 467 nm; emission wavelength: 538 nm.
Hoechst33342: Excitation wavelength: 350 nm; emission wavelength: 461 nm.
Green fluorescence: Observe under a microscope using the FITC filter cube, and stain the nuclei with Hoechst 33342 (Component D), then visualize under a fluorescence microscope equipped with a DAPI filter set.
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Fill in the product batch number for inquiry
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Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
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