EZ ECL Chemiluminescent Solution (Enhanced)
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AP34L014100mL198
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AP34L015500 mL898
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
EZ ECL Chemiluminescent Solution (Enhanced) is a highly sensitive HRP‑based chemiluminescent (ECL) substrate that enables users to detect proteins at the picogram level (~1×10⁻¹² g) in Western blotting assays. – 12 g) Extremely antibody‑efficient. The EZ ECL substrate delivers bright signals and picogram‑level detection sensitivity in Western blotting assays using horseradish peroxidase (HRP)-conjugated antibodies. This substrate is compatible with a wide range of membranes, blocking solutions, and antibody diluents, offering outstanding performance, broad compatibility, and excellent cost‑effectiveness to meet users’ Western blotting needs.
EZ ECL Characteristics of the substrate:
High sensitivity: detects picogram‑level protein bands on nitrocellulose or PVDF membranes.
Signal duration: Under optimized conditions, the blotting strip incubated with the substrate can sustain a detectable luminescent signal for 6 to 8 hours.
Recommended dilution concentration:
Primary antibody concentration: It is recommended to start with a 1 mg/mL stock solution at a dilution of 1:1,000–1:5,000, or 0.2–1 µg/mL.
Secondary antibody concentration: It is recommended to start with a 1 mg/mL stock solution diluted to 1:20,000–1:100,000, or to a final concentration of 10–50 ng/mL.
Order Information
Product Name
Item number
Specifications
EZ ECL Chemiluminescent Solution (Enhanced)
AP34L014
100 mL
EZ ECL Chemiluminescent Solution (Enhanced)
AP34L015
500 mL
Product ingredients
Component
AP34L014
AP34L015
Enhanced Type A Liquid
50 mL
250 mL
Enhanced Type B Liquid
50 mL
250 mL
Transportation and Save
Blue Ice Transport. Store at 4°C, protected from light and in a dry environment; shelf life: 24 months.
ECL Chemiluminescent Reagent Operation Demonstration Video
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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ECL Chemiluminescent Reagent Operation Demonstration Video
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