EZ ECL Femto Chemiluminescent Solution (Ultra-Sensitive)
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AP34L034100mL698
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AP34L035500 mL2698
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
EZ ECL Femo chemiluminescent solution is currently the highest‑sensitivity enhanced chemiluminescence (ECL) substrate for HRP, enabling users to achieve femtogram‑level protein detection in Western blotting assays (< 1× 10 -15 g ), extremely antibody‑efficient. West Femo substrate delivers bright signals and femtogram‑level detection sensitivity for Western blotting assays using horseradish peroxidase (HRP) conjugates. This ECL substrate is compatible with a wide range of membranes, blocking solutions, and antibody diluents, offering outstanding performance, broad compatibility, and excellent cost‑effectiveness to meet users’ Western blotting needs.
Characteristics of West Femo substrates:
High sensitivity: detects protein bands at the femtogram level on nitrocellulose or PVDF membranes.
Signal duration: Under optimized conditions, the blotting strip incubated with the substrate can sustain a detectable luminescent signal for 6 to 8 hours.
Recommended dilution concentration:
Primary antibody concentration: It is recommended to dilute the 1 µg/mL stock solution at a ratio of 1:5,000 to 1:100,000.
Secondary antibody concentration: It is recommended to dilute the 1 µg/mL stock solution 1:100,000 to 1:500,000.
Order Information
Product Name
Item number
Specifications
EZ ECL Femto Chemiluminescent Solution (Ultra-Sensitive)
AP34L034
100 mL
EZ ECL Femto Chemiluminescent Solution (Ultra-Sensitive)
AP34L035
500 mL
Product ingredients
Component Name
AP34L024
AP34L025
Ultra-sensitive type Liquid A
50 mL
250 mL
Ultra-sensitive type Solution B
50 mL
250 mL
Transportation and Storage
Blue Ice Transport. Store at 4°C, protected from light and in a dry environment; shelf life: 24 months.
ECL Chemiluminescent Reagent Operation Demonstration Video
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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ECL Chemiluminescent Reagent Operation Demonstration Video
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