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EZ ECL Chemiluminescent Solution (Standard Type)
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AP34L044100mL198
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AP34L045500 mL898
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
EZ ECL Chemiluminescent Solution (Standard Type) is a conventional‑sensitivity HRP‑based chemiluminescent (ECL) substrate that enables users to detect proteins at the 10 pg level (~1×10… -11 g) Extremely antibody‑efficient. The EZ ECL substrate delivers bright signals and picogram‑level detection sensitivity in Western blotting assays using horseradish peroxidase (HRP)-conjugated antibodies. This substrate is compatible with a wide range of membranes, blocking solutions, and antibody diluents, offering outstanding performance, broad compatibility, and excellent cost‑effectiveness to meet users’ Western blotting needs.
Characteristics of the EZ ECL substrate:
Conventional sensitivity: detects a 10 pg protein band on nitrocellulose or PVDF membranes.
Signal duration: Under optimized conditions, the blotting strip incubated with the substrate can sustain a detectable luminescent signal for 6 to 8 hours.
Recommended dilution concentration:
Primary antibody concentration: It is recommended to start with a 1 mg/mL stock solution at a dilution of 1:1,000–1:5,000, or 0.2–1 µg/mL.
Secondary antibody concentration: It is recommended to prepare a 1 mg/mL stock solution and dilute it to 1:5,000–1:20,000, or 2.5–10 ng/mL.
Order Information
Product Name
Item number
Specifications
EZ ECL Chemiluminescent Liquid (Standard Type)
AP34L044
100 mL
EZ ECL Chemiluminescent Liquid (Standard Type)
AP34L045
500 mL
Product ingredients
Component
AP34L044
AP34L045
Standard type A Liquid
50 mL
250 mL
Standard type B Liquid
50 mL
250 mL
Mode of transportation
Blue Ice Transport. Store at 4°C, protected from light and in a dry environment; shelf life: 24 months.
ECL Chemiluminescent Reagent Operation Demonstration Video
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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ECL Chemiluminescent Reagent Operation Demonstration Video
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Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
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