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Geneticin (G418)
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AB11L2341g780
- Product Description
- Product FAQ
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Product Description
G418 Sulfate (G418 sulfate), also known as Geneticin, G418, G 418, or G‑418, is an aminoglycoside antibiotic structurally similar to Gentamycin B1, derived from Micromonospora rhodorangea. It is commonly used for selecting eukaryotic or prokaryotic cells—whether polyclonal or monoclonal—that express the neo gene. G418 is employed not only for screening stable cell lines but also for maintaining them. The 50 mg/mL formulation of this product is sterile-filtered and ready for direct use in cell culture.
In bacteria, yeast, protozoa, nematodes, higher plants, and mammals, geneticin inhibits or kills cells by suppressing protein synthesis. Its mechanism of action involves binding to 70S and 80S ribosomes, thereby blocking peptide chain elongation and disrupting protein synthesis. The resistance gene—primarily the neo gene—is carried on transposons Tn601 (903) or Tn5 (of bacterial origin) and can be expressed in eukaryotic cells. By introducing these resistance genes into cells via genetic recombination, the cells acquire the ability to produce the aminoglycoside phosphotransferase (APH(3)II), conferring resistance to G418 and enabling the selection and maintenance of prokaryotic or eukaryotic cells harboring the resistance gene.
In mammalian cells, when the resistance gene neo is integrated into the eukaryotic genome, it encodes and expresses the amino‑glycoside 3′‑phosphotransferase (APH(3)II). This enzyme covalently modifies the amino or hydroxyl groups of G418, thereby inhibiting the interaction between the antibiotic and the ribosome and rendering the antibiotic inactive. This property confers resistance, allowing cells to grow on selective media containing G418. When performing stable transfection screening, it is necessary to establish a dose–response curve (or kill curve) to determine the minimum effective concentration required to kill non‑resistant cells.
In plant cells, resistance is conferred by incubating the cells with an antibiotic‑resistant plasmid carrying the nptII gene. The nptII gene also encodes aminoglycoside phosphotransferase, an enzyme that inactivates several antibiotics, including G418, kanamycin, and paromomycin.
G418 and neomycin are both aminoglycoside antibiotics that can be inactivated by the product of the Neo gene; therefore, they can both be used to select cells harboring the Neo resistance gene. Cells selected with neomycin can be similarly selected using G418.
Product ingredients
Product Name
Specifications
Geneticin (G418)
1 g
Transportation and Storage
Store at room temperature. Store at 4°C; shelf life: 36 months.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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Q: What is the vitality value?
A: The minimum activity is 650 µg/mg. Activity levels may vary between batches. Upon receipt, please provide the batch number to our after-sales team, and we will verify the specific activity level for that batch.
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Fill in the product batch number for inquiry
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Fill in the product batch number for inquiry
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