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BCA Protein Quantitation Kit
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AP12L025500T198
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
The BCA (bicinchoninic acid) assay is currently one of the most widely used methods for determining protein concentration. It is based on the biuret reaction, in which proteins reduce Cu²⁺ to Cu⁺ under alkaline conditions. + , Cu + A purple-colored complex is formed with the BCA reagent, exhibiting a high absorbance at 562 nm; the amount of this reaction product is directly proportional to the protein concentration. By measuring the absorbance at 562 nm and comparing it to a standard curve, the concentration of the protein sample can be determined. This method is rapid, sensitive, stable, and reliable, with a very low coefficient of variation across different protein types. The kit includes a stable protein standard for constructing the standard curve.
The BCA protein assay kit can be used for both tube‑based and microplate‑based assays. The tube method requires a relatively large protein sample (100 μL) and working reagent (2 mL), with a protein-to‑working‑reagent ratio of 1:20 (v/v). The measurable protein concentration range is 20–2000 μg/mL; with the enhanced protocol, detection down to 5 μg/mL is possible. The microplate method is simple and convenient, requiring only a small volume of protein sample (10–25 μL) and working reagent (200 μL), at a protein-to‑working‑reagent ratio of either 1:20 (v/v) or 1:8 (v/v). The measurable protein concentration range is 20–2000 μg/mL, and the enhanced protocol allows detection down to 5 μg/mL.
Our company offers the BCA protein assay kit, with 50 assays using the colorimetric method and 500 assays using the microplate method.
Product ingredients
Item number
Component
Specifications
AP12L025
BCA Reagent A
100mL
BCA Reagent B
5mL
BSA standard solution 2 mg/mL
2*1 mL
Transportation and Save
Store at room temperature. Keep refrigerated at 4°C; shelf life is 12 months.
BCA Assay Interferent Compatibility Table
Name of interfering substance
Tolerable concentration
Name of interfering substance
Tolerable concentration
Sodium bica rbonate
100 mM
BugBuster Protein Extraction Reagent ( Cat. No. 70584)
n interference
( undiluted )
Sodium phos phate
25 m M
Calcium chloride ( in TBS, pH8.0 )
10 mM
2-Me rcaptoethanol
0.01%
Cell Tytic B Reagent
nointerference
( undiluted )
G glycerol ( pure )
10%
Cesium m bicarbonate
100 mM
Glycine - HCl , pH 2.8
100 mM
CHAPS
5%
HEP ES
100 mM
Cobalt chloride ( in TBS, pH8.0 )
0.8 mM
Hydrochloric acid
100 mM
CytoBuster Protein Extraction Reagent ( Cat. No . 71009)
n interference
( undiluted )
Leupeptin
10 mg/L
Deoxycholic acid
5%
Nickel chloride ( in TBS, pH8.0 )
10 mM
Dithioerythritol ( DTE )
1 mM
Nonidet P-40 (NP-40)
5% (w/ v )
Dithiothreitol ( DTT
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Fill in the product batch number for inquiry
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BCA Protein Quantitation Procedure Demonstration Video
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Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
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