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LyGreen HRM-specific dye (20× in water)
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AN19L0211 mL850
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AN19L0225*1 mL3300
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Product Description
LyGreen nucleic acid dye is a novel, saturation‑binding fluorescent dye for double‑stranded DNA. It represents a next‑generation fluorescent dye designed for HRM analysis and can also be used in real‑time quantitative PCR (qPCR). Structurally, it consists of two monomeric dye molecules linked by an elastic arm, forming a dimer. In the absence of DNA, this dimer adopts a non‑fluorescent cyclic conformation; upon DNA binding, it reverts to a random‑coil shape and emits intense green fluorescence.
Compared with other fluorescent dyes on the market, such as SYBR Green I, LyGreen offers the following advantages:
(1) High sensitivity : When used at the recommended concentration, it delivers a stronger signal than SYBR Green I.
(2) Accurate : When bound to double-stranded DNA, the dye remains immobile during conformational changes, ensuring that the fluorescence signal accurately reflects alterations in double-stranded DNA. Excess dye forms dimers rather than remaining free, resulting in significantly lower inhibition of PCR by LyGreen compared to SYBR Green I. Consequently, qPCR assays using LyGreen can employ rapid PCR cycling protocols.
(3) Good stability : It remains stable under normal storage, handling, and PCR conditions. The dye in buffer solution can be safely stored at room temperature or in the refrigerator and is compatible with repeated freeze–thaw cycles.
(4) Safety Cell membrane permeability assays indicate that LyGreen exhibits virtually no cell membrane permeability.
(5) Compatibility : With a spectral profile similar to that of SYBR Green I, it requires no adjustment of spectral settings or modification of experimental protocols when used with qPCR instruments from different manufacturers. After qPCR is completed, no additional nucleic acid dye needs to be added; the results can be directly visualized and analyzed on a gel imaging system.
(6) Good repeatability (As shown in Figure 1): A specially designed dye exhibits fluorescence signal release that, under identical reaction conditions, depends solely on the concentration of double-stranded DNA, with perfect linearity.
Figure 1. LyGreen dye used for HRM analysis of the FecB gene in sheep.
The HRM analysis instrument is the LightCycler® 480 real-time PCR system (Roche, Germany).
Product ingredients
Product Name
Item number
Specifications
LyGreen HRM-specific dye (20× in water)
AN19L021
1 mL
LyGreen HRM-specific dye (20× in water)
AN19L022
5*1 mL
Transportation and Storage
Blue ice transport. Store at -20°C, dry and protected from light; shelf life: 12 months.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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